Simultaneous fluorescence detection of fecal urobilins and porphyrins by reversed-phase high-performance thin-layer chromatography.

نویسندگان

  • C W Lam
  • C K Lai
  • Y W Chan
چکیده

J Med 1996;334:759–62. 9. Boushey CJ, Beresford SA, Omenn GS, Motulsky AG. A quantitative assessment of plasma homocysteine as a risk factor for vascular disease. Probable benefits of increasing folic acid intakes. JAMA 1995;274:1049–57. 10. Ubbink JB, Vermaak WJ, van der Merwe A, Becker PJ. The effect of blood sample aging and food consumption on plasma total homocysteine levels. Clin Chim Acta 1992;207:119–28. 11. Vester B, Rasmussen K. High performance liquid chromatography method for rapid and accurate determination of homocysteine in plasma and serum. Eur J Clin Chem Clin Biochem 1991;29:549–59. 12. Fiskerstrand T, Refsum H, Kvalheim G, Ueland PM. Homocysteine and other thiols in plasma and urine: automated determination and sample stability. Clin Chem 1993;39:263–71. 13. te Poele Pothoff MT, van den Berg M, Franken DG, Boers GH, Jakobs C, de Kroon IF, et al. Three different methods for the determination of total homocysteine in plasma. Ann Clin Biochem 1995;32:218–20. 14. Stabler SP, Marcell PD, Podell ER, Allen RH. Quantitation of total homocysteine, total cysteine, and methionine in normal serum and urine using capillary gas chromatography-mass spectometry. Anal Biochem 1987;162: 185–96. 15. Kittner SJ, Malinow MR, Seipp MJ, Upson B, Hebel JR. Stability of blood homocyst(e)ine under epidemiological field conditions. J Clin Lab Anal 1995;9:75–6. 16. Møller J, Rasmussen K. Homocysteine in plasma: stabilization of blood samples with fluoride. Clin Chem 1995;41:758–9.

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عنوان ژورنال:
  • Clinical chemistry

دوره 44 2  شماره 

صفحات  -

تاریخ انتشار 1998